Recombinant Mouse Leptin
Background
Leptin is a newly discovered protein encoded by the obese gene in adipose cells. Administration of recombinant leptin into animals decreases food intake, increases energy expenditures and promotes weight loss. It is also being studied for possible roles in reproduction and hematopoiesis.
Specifications
| Package Size: | 100 µg |
| Source: | Expressed in E. coli |
| Molecular Weight: | Contains 147 amino acid residues and has a predicted molecular mass of approximately 16 kDa. |
| Purity: | >97% as determined by SDS-PAGE and visualized by silver stain. |
| Endotoxin Level: | <0.1 ng per 1 µg as determined by the LAL method. |
| Activity: | Produces a dose-dependent reduction in body weight and food intake when tested in obese mice (ob/ob) given daily intraperitoneal injections. |
| Formulation: | Lyophilized from a 1 mg/ml solution of 55 µl HCL (15mM), 33 µl NaOH (7.5 mM), and 22 µl 0.1% Triton X-100. |
| Reconstitution: | Add 100 µl of sterile, double-distilled water to obtain 1 mg/ml. Further dilute as required in a matrix of choice. When possible, it is best to utilize dilute (approximately 10 mM), mildly basic (approximately pH9) buffers containing 0.05% of Triton X-100 to reduce non-specific absorption. |
| Storage: | Lyophilized leptin is stable for at least six months at -20 °C. Upon reconstitution, the 1 mg/ml solution can be stored at 4 °C for one month or at -20 °C for at least three months. Avoid repeated (>5) freeze-thaw cycles. |
For research use only.
Not for use in humans or clinical diagnostics.