Human Insulin-Specific RIA Kit

This kit selectively measures immunoreactive insulin and is the assay of choice when proinsulin levels are elevated or variable. Crossreactivity is less than 0.2% to intact human proinsulin and the primary circulating split form (Des 31, 32). The kit includes all components (standards, antibody, tracer, quality controls, precipitating reagents and buffer) necessary to complete a RIA.

ASSAY CONDITIONS: Overnight, equilibrium incubation at room temperature. Sample volume: £ 100 µl serum or plasma.

LABEL: 125I-Insulin (monoiodinated, HPLC purified, 25,000 CPM/tube)

 

SPECIFICITY:
Human Insulin
100%
Intact Human
Proinsulin
<0.2%
Des 31, 32 HPI
<0.2%
Des 64, 65 HPI
76%
Porcine Insulin
100%
Canine Insulin
100%
Bovine Insulin
62%
Rat Insulin
<0.1%
IGF-I
*
Glucagon
*
Somatostatin
*
Pancreatic
Polypeptide
*
* Not Detectable

PERFORMANCE:

ED80 = 6 ± 1 µU/ml
ED50 = 26 ± 1 µU/ml
ED20 = 103 ± 6 µU/ml