Human C-Peptide RIA Kit

An overnight assay using serum or plasma with very low crossreactivity to human proinsulin. No extraction of sample is required.

LABEL: 125I-Human C-Peptide

PERFORMANCE:

ED80 = 0.3 ± 0.1 ng/ml
ED50 = 1.1 ± 0.1 ng/ml
ED20 = 4.1 ± 0.4 ng/ml 

ASSAY CONDITIONS: Overnight, equilibrium incubation at 4°C Sample volume - 100 μl serum, plasma, or tissue culture media

STANDARD CURVE RANGE:   0.1 - 5 ng/mL

SENSITIVITY IN TISSUE CULTURE/SERUM/PLASMA:   0.1 ng/mL

 

SPECIFICITY:
Human C-Peptide
100%
Human Proinsulin
<4.0%
Human Insulin
*
Glucagon
*
Somatostatin
*
Pancreatic Polypeptide
*
Monkey C-Peptide
90%
Rat C-Peptide
*
Porcine C-Peptide
*
* Not Detectable

ACCURACY:   96 - 101%


PRECISION:   Intra-assay 3.4 - 6.4%
                          Inter-assay 2.4 - 9.3%

  

• All components are included in each kit (standards, antibody, tracer, quality controls, precipitating reagent and buffers) to complete a RIA.