Active Ghrelin ELISA Kit

Ghrelin, a novel growth hormone releasing peptide is an acylated peptide that stimulates the release of growth hormone from pituitary. It was isolated from rat stomach and the structure was determined as a small peptide consisting of 28-amino acids by Dr. Kenji Kangawa (National Cardiovascular Center in Japan). The Ser3 residue of Ghrelin is modified by n-octanoic acid, a modification necessary for hormonal activity. Ghrelin has been implicated in regulation of hunger and long-term weight gain/loss.

Ghrelin is the endogenous ligand for the growth hormone secretagogue (GHS) receptor and has potent growth hormone-releasing activity. This peptide may constitute a new regulatory mechanism for GH-release. It is conceivable that Ghrelin may also have other functions in some tissues in addition to pituitary, because the GHS receptor is expressed in heart, lung, pancreas, intestine, and adipose tissue.

The Active Ghrelin ELISA kit recognizes the octanoyl-modified portion of Ghrelin and is manufactured using the highly specific antibody pairs generated by Dr. Kangawa and by following his protocol. (patent pending: PCT WO 01/07475 A1)

This kit is for research purposes only.

 

ASSAY CONDITIONS:  
Room temperature, 3.5 hour assay

SAMPLE SIZE:
50 µL / Plasma, Culture Media, CSF; or any biological fluid

SENSITIVITY: 
The lowest level of Active Ghrelin that can be detected by this assay is 1 fmol/mL when using a 50 µL sample size.

 

 

PRECISION: 
Intra-assay 3.5% - 5.5%
Inter-assay 2.6% - 4.0%

LINEARITY OF DILUTION: 
94 - 104%

CROSS REACTIVITY: 

Analyte Cross reactivity
Human Active Ghrelin 100%
Rat Active Ghrelin 100%
Human Desacyl-Ghrelin <0.1%
Rat Desacyl-Ghrelin <0.1%