Mouse Resistin RIA Kit

Mouse Resistin1 is a new member of an ever increasing family of Adipokines and is also referred to as ADSF (adipocyte specific secretory factor) 2 and FIZZ3 (found in inflammatory zone) 3. Resistin is a secretory protein from adipocytes. Mouse Resistin is a 94 amino acid protein with 11 cystein residues and circulates as a dimer of 2 identical peptides linked by disulfide bond 4. Mouse Resistin shares ~ 60 homology with human Resistin. In addition to mouse Resistin, two other Resistin like molecules, RELM-alpha and RELM-beta with 29 and 37 percent sequence homology at the protein level are expressed in white adipose tissue and the gut, respectively 5. Mouse Resistin is implicated as the missing link between obesity and insulin resistance and type 2 diabetes. Mouse Resistin levels increase with obesity in various mouse models. Exogenous administration of mouse Resistin impairs glucose tolerance and insulin action. Measurement of mouse Resistin levels in circulation under different patho-physiological, nutritional and genetic manipulations should result in better understanding of the etiology of type 2 diabetes and obesity.

LINCO's Mouse Resistin RIA is the first available assay to specifically measure resistin levels in mouse serum/plasma or tissue culture media..

ASSAY CONDITIONS: Three day assay at room temperature (22ºC). Sample volume: 50 µl. Plasma, serum or tissue culture medium.


LABEL:  125I-Mouse Resistin (~20,000 CPM/tube)


Mouse Resistin 100%
Human Resistin *
Mouse RELM-beta < 0.2%
Rat Growth Hormone *
Mouse Adiponectin *
Rat Insulin *
Mouse Leptin 0.5%
*  Not detectable 


ED80 = 2.23 ± 0.15 ng/ml
ED50 = 6.15 ± 0.25 ng/ml
ED20 = 16.36 ± 0.68 ng/ml 


  1. Steppan C.M., et. Al. The hormone resistin links obesity and diabetes. Nature 409: 307-311, 2001.
  2. Kim, K-H. A cystein-rich adipose tissue specific secretory factor inhibits adipocyte differentiation. J. Bio. Chem. 276: 11252-11256, 2001.
  3. Holcomb, I.N. et. al. FIZZ1, a npvel cystein-rich secreted protein associated with pulmonary inflammation, defines a new gene family. Embo J. 19 40646-4055, 2000.
  4. Banerjee, R.R. and Lazar, M.A. Dimerization of resistin and resistin-like molecules is determined by a single cystein. J. Bio. Chem. 276: 25970-25973, 2001.
  5. Steppan C.M. et. al. A family of tissue-specific resistin-like molecules. Proc. Nat. Acad. Sci. 98: 502-506-,