Mouse Resistin RIA Kit
Mouse Resistin1 is a new member of an ever increasing family of Adipokines and is also referred to as ADSF (adipocyte specific secretory factor) 2 and FIZZ3 (found in inflammatory zone) 3. Resistin is a secretory protein from adipocytes. Mouse Resistin is a 94 amino acid protein with 11 cystein residues and circulates as a dimer of 2 identical peptides linked by disulfide bond 4. Mouse Resistin shares ~ 60 homology with human Resistin. In addition to mouse Resistin, two other Resistin like molecules, RELM-alpha and RELM-beta with 29 and 37 percent sequence homology at the protein level are expressed in white adipose tissue and the gut, respectively 5. Mouse Resistin is implicated as the missing link between obesity and insulin resistance and type 2 diabetes. Mouse Resistin levels increase with obesity in various mouse models. Exogenous administration of mouse Resistin impairs glucose tolerance and insulin action. Measurement of mouse Resistin levels in circulation under different patho-physiological, nutritional and genetic manipulations should result in better understanding of the etiology of type 2 diabetes and obesity.
LINCO's Mouse Resistin RIA is the first available assay to specifically measure resistin levels in mouse serum/plasma or tissue culture media..
ASSAY CONDITIONS: Three day assay at room temperature (22ºC). Sample volume: 50 µl. Plasma, serum or tissue culture medium.
STANDARD CURVE RANGE: 0.78 - 50 ng/mL
LABEL: 125I-Mouse Resistin (~20,000 CPM/tube)