Mouse Serum Adipokine Panel

Adipose tissue is not only a site of energy storage, but also an active endocrine organ that secretes a number of hormones and cytokines including leptin, resistin, adiponectin, IL-6, TNFa, MCP-1, PAI-1 and others.  These adipocyte-derived proteins (adipocytokines/adipokines) play critical roles in body energy homeostasis and metabolism.  This multiplex assay kit manufactured by LINCO Research, Inc. is to be used for the simultaneous quantification of the following 7 mouse adipokines in any combination: Insulin, Leptin, Resistin, IL-6, TNFα, MCP-1, and PAI-1 (total).  This kit is for research purposes only.

All components are included in each kit (capture antibody immobilized on microspheres, detection antibody cocktail, standards cocktail, quality controls, streptavidin-PE, wash buffer, assay buffer, 96-well microtiter filter plate) in sufficient quantity to run 39 samples in a 96-well microtiter plate. 

ASSAY CONDITIONS:  
Overnight assay; 10 µl sample volume.

STANDARD CURVE RANGE:  
16 - 50,000 pg/mL

PRECISION:  
Intra-assay (%CV) < 4.5%
Inter-assay (%CV) <10.3%

CROSS REACTIVITY:  
The antibody pairs used in this assay are specific and there is no significant cross-reaction within this panel.
ACCURACY/RECOVERY:
 

In Serum Matrix
   
Leptin 101%
Resistin 104%
IL-6 105%
TNFa 95%
MCP-1 99%
PAI-1 105%
Insulin 95%
SENSITIVITY:
 

In Serum Matrix
   
Leptin 16.71
Resistin 1.79
IL-6 1.89
TNFa 4.39
MCP-1 14.4
PAI-1 16.4
Insulin 42.2

 
 

This kit is for research only and not for use as a clinical or diagnostic tool.