Rat/Mouse Insulin ELISA Kit

This kit is used for the non-radioactive quantification of insulin in mouse and rat sera.  Plasma samples may also be used but application to samples of other biological fluids may need validation by the user.  Each kit is sufficient to run one 96-well plate including, in duplicates, background, 6 rat insulin standards, 2 quality controls and 39 unknown samples. This kit is for research purpose only.

SPECIFICITY:
Rat Insulin 100%
Porcine Insulin 102%
Bovine Insulin 78%
Ovine Insulin 106%
Human Insulin 106%
Human Proinsulin 52%
Des(64,65) Human Proinsulin 101%
Des(31,32) Human Proinsulin 69%
Porcine Proinsulin 57%
Bovine Proinsulin 56%
Human IGF-I *
Human IGF-II *
Porcine Glucagon *
Human C-Peptide *
Rat C-Peptide *
Rat Leptin *
Mouse Leptin *

* Not detectable at concentrations up to 120 nM

 ASSAY CONDITIONS:  
Room temperature, 3 hour assay using 10 µl sample size.

DYNAMIC RANGE:  
0.2 ng/ml to 10 ng/ml insulin

PERFORMANCE:  
ED50 = 1.57  ±  0.02 nM, n=3

PRECISION:  
Intra-assay  1.2 - 8.4%
Inter-assay  6.0 - 17.9%

LINEARITY OF DILUTION:  
79 - 133%

SPIKING RECOVERY:  
83 - 102%