Human Serum Adipokine, Panel A: HADK1-61K-A
Human Serum Adipokine, Panel B: HADK2-61K-B
Adipose tissue is not only a site of energy storage, but also an active endocrine organ that secretes a number of hormones and cytokines including leptin, resistin, adiponectin, IL-6,TNFα, MCP-1, PAI-1 and others. These adipocyte-derived proteins (adipocytokines/adipokines) play critical roles in body energy homeostasis and metabolism. LINCO has developed the following multiplex immunoassays for simultaneous quantitative determination of several adipokines in human serum or adipocytes, using Luminex xMAP technology.
All components are included in each kit (capture antibody immobilized on microspheres, detection antibody cocktail, standards cocktail, quality controls, streptavidin-PE, wash buffer, assay buffer, serum matrix, bead diluent 96-well microtiter filter plate) in sufficient quantity to run 38 samples
in duplicate in a 96-well microtiter plate.
1) Human Adipocyte Panel (Catalog No. HADCYT-61K): This kit is used to measure Leptin, Resistin, Adiponectin, IL-1β, IL-6, IL-8,TNFα, MCP-1, PAI-1, HGF, and NGF in adipocyte extracts or culture medium samples.
2) Human Serum Adipokine Panel A (Catalog No. HADK1-61K-A): This kit is used to measure Adiponectin, Resistin, and PAI-1 (total or active) in human serum or plasma. (1:400 dilution is required)
3) Human Serum Adipokine Panel B (Catalog No. HADK2-61K-B): This kit is used to measure Leptin, IL-1β, IL-6, IL-8,TNFα, MCP-1, HGF, and NGF
Insulin in serum and plasma samples.
|
ASSAY CONDITIONS:
Overnight assay.
25 µL sample volume
Serum and plasma samples need 1:400 dilution for Panel A.
CROSS REACTIVITY:
Each antibody pair in a panel is highly specific and displays insignificant cross-reactivity to other analytes in the panel.
STANDARD CURVE RANGE:
0.64 - 10,000 pg/mL IL-1β, IL-6, IL-8, TNFα, MCP-1, NGF
3.2 - 50,000 pg/mL Resistin, PAI-1, HGF, Insulin
16 - 250,000 pg/mL Leptin, Adiponectin
| ACCURACY / RECOVERY in buffer matrix: |
| |
Adipocyte
Panel
|
Serum Adipokine
Panel A
|
Serum Adipokine
Panel B |
| Leptin |
95.7%
|
-
|
99.8% |
| Resistin |
90.2%
|
100.8%
|
- |
| Adiponectin |
95.5%
|
98.9%
|
- |
| IL-6, IL-1b, IL-8 |
92.1%
|
-
|
93.9% |
| TNF-a |
94.9%
|
-
|
91.5% |
| MCP-1 |
92.0%
|
-
|
90.2% |
| PAI-1, HGF |
90.6%
|
99.9%
|
96.3% |
| Insulin, NGF |
89.1%
|
-
|
89.2% |
|
| SENSITIVITY: (Minimum Detectable Concentrations, MinDC, pg/mL): |
| |
Adipocyte
Panel
|
Serum Adipokine
Panel A |
Serum Adipokine
Panel B |
| Leptin |
27.4
|
-
|
85.4 |
| Resistin |
4.5
|
6.7
|
- |
| Adiponectin |
80.3
|
145.4
|
- |
| iL-6, IL-1b, IL-8 |
0.11
|
-
|
0.1 |
| TNF-a |
0.11
|
-
|
0.2 |
| MCP-1 |
0.5
|
-
|
0.14 |
| PAI-1, HGF |
1.6
|
1.3
|
19.2 |
| Insulin, NGF |
1.0
|
-
|
2.5 |
PRECISION: Intra-assay 1.4-7.9%
Inter-assay < 21%
LINEARITY OF DILUTION: 90-116%
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|
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