Lispro Insulin RIA Kit
This kit measures lispro insulin (Humalog©) in serum, plasma, or other biological fluids without crossreactivity to native human insulin or proinsulin. Lispro insulin is a synthetic human insulin analog with inverted B-chain amino acids in positions 28 (proline) and 29 (lysine). The kit includes all components (standards, matrix, antibody, tracer, quality controls, precipitating reagent and buffers) necessary to complete a RIA.
ASSAY CONDITIONS: Overnight, equilibrium incubation at room temperature. Sample volume <100 µl serum, plasma, or tissue culture media.
LABEL: 125I-Lispro Insulin (monoiodinated, HPLC purified, 18,000 CPM/tube).
ED80 = 9.75 ± 0.78 µU/ml
ED50 = 35.8 ± 1.90 µU/ml
ED20 = 136.0 ± 5.0 µU/ml