Human Leptin "Dual Range" ELISA Kit
Leptin is synthesized in adipocytes as a 16kD molecule and the circulating level is directly proportional to the total amount of fat in the body. It binds to its receptors in the hypothalamus and plays an active role in regulation of food intake, energy expenditure and reproductive function.
This kit is used for the non-radioactive quantification of human serum, plasma and other biological media. One kit is sufficient to measure 37 unknown samples in duplicate.
*All components are included (standards, quality controls, wash buffer, assay buffer, substrate and stop solutions, capture antibody immobilized plate, biotinylated detection antibody, and enzyme linked streptavidin) necessary to complete a 96-well plate ELISA
This kit can be used for measurements of Leptin in the normal physiological range (0.5 - 100 ng/mL) or for samples with low levels of Leptin where higher sensitivity is required. An alternate protocol is used for higher sensitivity measurements.
ASSAY CONDITIONS: 3.5 hour, room temperature. 25 µL sample size (Sensitive Assay: 50 µL)