Rat Cytokine/Chemokine Immunoassay Panel
This multiplex assay kit manu factured by LINCO Research is to be used for the simultaneous quantification of the following twenty four rat cytokines/chemokines in any combination: Eotaxin, G- CSF, GM- CSF, G RO / K C , IFNγ, IL- 1α, IL- 1β, IL- 2, IL- 4, IL- 5, IL- 6, IL- 9,
IL- 10, IL- 12( p70), IL- 13, IL- 17, IL- 18, IP- 10, Leptin, MCP- 1, MIP- 1α, RANTES,
TNFα and VEGF. This kit may be used for the analysis of the above cytokines and chemokines in rat serum, plasma, other rat biological fluids, tissue/ cell extracts, or cell culture supernatants.
All components are included in each kit (capture antibody immobilized on microspheres, detection antibody, standards, quality controls, streptavidin- PE, wash buffer, assay buffer, 96 - well microtiter filter plate, serum matrix) and are sufficient to run 38 samples ( serum, plasma or tissue culture medium) in duplicate in a 96-well microtiter plate.
ASSAY CONDITIONS: 5 μL serum/plasma, 25 μL or less for tissue culture supernatant
Overnight assay
STANDARD CURVE RANGE: 24.4 to 100,000 pg/mL for rat Leptin;
4.88 to 20,000 pg/mL for all other analytes
SAMPLE REQUIREMENT: 25 μL of 1:5 Diluted Serum or Plasma 25 μL of cell culture supernatant (Users need to determine appropriate dilutions.)
SENSITIVITY: 1.31 – 54.42 pg/mL; Refer to kit protocol for sensitivities for individual cytokines/chemokines.
CROSS REACTIVITY: Not detectable within the panel
ACCURACY : 83 – 109%
PRECISION: Intra- assay CV: 3.8 – 12.3%
Inter- assay CV: 5.9 – 20.3%
LINEARITY OF DILUTION: Ranges: 82.2 to 141.1%
ANALYTES AVAILABLE:
Order any combination of analytes, or a 14-plex or 24-plex premixed panel.
| 14-PLEX | 24-PLEX | |
| IL-1a | IL-1a | IL-1a |
| IL-1b | IL-1b | IL-1b |
| IL-2 | IL-2 | IL-2 |
| IL-4 | IL-4 | IL-4 |
| IL-5 | IL-5 | IL-5 |
| IL-6 | IL-6 | IL-6 |
| IL-9 | IL-10 | IL-9 |
| IL-10 | IL-12(p70) | IL-10 |
| IL-12(p70) | IL-18 | IL-12(p70) |
| IL-13 | GM-CSF | IL-13 |
| IL-17 | GRO/KC | IL-17 |
| IL-18 | IFNg | IL-18 |
| Eotaxin | MCP-1 | Eotaxin |
| G-CSF | TNFa | G-CSF |
| GM-CSF | GM-CSF | |
| GRO/KC | GRO/KC | |
| IFNg | IFNg | |
| IP-10 | IP-10 | |
| Leptin | Leptin | |
| MCP-1 | MCP-1 | |
| MIP-1a | MIP-1a | |
| RANTES | RANTES | |
| TNFa | TNFa | |
| VEGF | VEGF |