Human Proinsulin RIA Kit
This human leptin assay has been developed to measure human leptin in plasma, serum, and tissue culture media. It is a completely homologous assay since the antibody was raised against highly purified human leptin and both the standard and tracer are prepared with human leptin. All components are included (standards, antibody, tracer, quality controls, precipitating reagents and buffer) necessary to complete a RIA.
ASSAY CONDITIONS: Overnight, equilibrium incubation at 4°C. Sample volume: £ 100 µl serum, plasma, or tissue culture media.
LABEL: 125 I-Human Leptin (HPLC purified, 18,000 CPM/tube).
ED80 = 1.3 ± 0.2 ng/ml
ED50 = 6.0 ± 1.0 ng/ml
ED20 = 29.3 ± 5.6 ng/ml