Multi-Species Leptin RIA Kit

This Multi-Species Leptin assay has been developed to quantitate leptin in plasma or serum from many animals. The antibody used in this kit was raised against human leptin but displays broad crossreactivity to leptin molecules of many, but not all, species. All components are included (standards, antibody, tracer, quality controls, precipitating reagents and buffer) necessary to complete a RIA.

ASSAY CONDITIONS: Two-day, disequilibrium incubation at 4°C. Sample volume: £ 100 µl serum or plasma.

LABEL: 125 I-Human Leptin (HPLC purified, 18,000 CPM/tube)

 

SPECIFICITY:
Human Leptin
100%
Porcine Leptin
67%
Rat Leptin
61%
Mouse Leptin
73%
Canine Leptin
3%
Human Insulin
*
Human Proinsulin
*
Human C-Peptide
*
Rat Insulin
*
Glucagon
*
IGF-I
*
* Not Detectable

PERFORMANCE:

ED80 = 2.0 ± 0.07 ng/ml HE**
ED50 = 5.8 ± 0.20 ng/ml HE
ED20 = 17.4 ± 0.77 ng/ml HE
** Human Equivalent