Human CVD Biomarker Panel 3

This multiplex assay kit manufactured by LINCO Research can be used for the simultaneous quantification of the following analytes in any combination: IL-1β, IL-6, IL-8, IL-10,IFNγ,MCP-1,TNFα,NT-proBNP, and VEGF. This kit can be used for the analysis of the above analytes in tissue extract, cell/tissue culture samples, and human serum or plasma samples.

ASSAY CONDITIONS:
25 μL sample volume
2 hour assay or overnight incubation for increased sensitivity
No dilutions required for serum or plasma samples

STANDARD CURVE RANGE:
3.2 to 50,000 pg/ml for NT-proBNP
0.64 to 10,000 pg/ml for IL-1β, IL-6, IL-8, IL-10, IFNγ, MCP-1,TNFα, and VEGF

SENSITIVITY IN
TISSUE CULTURE/SERUM/PLASMA (MinDC*):

IL-1β                   0.33 pg/ml
IL-6                     0.67 pg/ml
IL-8                     0.50 pg/ml
IL-10                   0.30 pg/ml
IFNγ                    0.40 pg/ml
MCP-1                0.59 pg/ml
NT-proBNP      11.50 pg/ml
TNFα                  0.22 pg/ml
VEGF                 1.52 pg/ml

*Minimum Detectable Concentration

CROSS REACTIVITY:
No detectable cross reactivity within the panel    

ACCURACY:

IL-1β                             99.6%
IL-6                             104.8%
IL-8                             101.2%
IL-10                             97.9%
IFNγ                            101.3%
MCP-1                          96.5%
NT-proBNP                102.6%
TNFα                            96.7%
VEGF                            99.2%

PRECISION:
Intra-assay           8.2 - 11.4%
Inter-assay           6.9 - 13.7%