Total Human Amylin ELISA Kit
Amylin is a 37 amino acid hormone that is co-secreted with insulin from pancreatic beta cells in response to hyperglycemia. Amylin appears to work in concert with insulin to regulate plasma glucose concentrations in the bloodstream, suppressing the postprandial secretion of glucagon and slowing the rate of gastric emptying. Thus, people with diabetes have a deficiency in the secretion of amylin that parallels the deficiency in insulin secretion, resulting in an excessive inflow of glucose into the bloodstream during the postprandial period.
The Total Human Amylin ELISA is a monoclonal antibody-based sandwich immunoassay for determining total amylin levels in human plasma. The capture antibody recognizes both the reduced human amylin and human amylin acid (deamidated amylin) but not the 1-20 fragment of human amylin. The detection antibody binds to reduced or unreduced human amylin but not amylin acid and is complexed with streptavidin-alkaline phosphatase. The substrate, 4-Methylumelliferyl Phosphate (MUP), is applied to the completed sandwich and the fluorescent signal, monitored at 355 nm/460nm, is proportional to the amount of amylin present in the sample.
Linco's Total Human Amylin ELISA Kit is validated for the measurement of total amylin from EDTA human plasma samples. It has sensitivity to 1 pM and a standard range of 1-100 pM.
ASSAY CONDITIONS: All incubations are at room temperature. 1 hour primary incubation on a microplate shaker. 2 hour secondary incubation on a microplate shaker. 1/2 hour substrate development. 50 µl sample volume per well.
ED80 = 82 ± 2 pM
ED50 = 54 ± 5 pM
ED20 = 26 ± 3 pM