Human Total Proinsulin ELISA Kit

This Human Total Proinsulin ELISA kit is used for the non-radioactive quantification of human total proinsulin in serum and plasma.  This kit has 100% cross reactivity to intact human proinsulin and its major processed intermediate des(31,32) proinsulin, 81% cross reactivity to another processed intermediate des(64,65) proinsulin, in serum and plasma.  One kit is sufficient to measure 38 unknown samples in duplicate.  This kit has no cross reactivity to human insulin.  This kit is for research purposes only.

SENSITIVITY IN TISSUE CULTURE/SERUM/PLASMA: 
The lowest level of Proinsulin that can be detected by this assay is 0.5 pM when using a 20 µL sample size.

 

ASSAY CONDITIONS:  
2.5 hour assay
20 µl sample size.
SPECIFICITY:
Human Insulin *
Intact Human Proinsulin 100%
Des(64,65) Human Proinsulin 81%
Des(31,32) Human Proinsulin 100%
Porcine Proinsulin ~1%
Bovine Proinsulin *
Human C-Peptide *
Rat C-Peptide *
Human Leptin *
Rat Leptin *
Glucagon *
* Not Detectable at concentrations up to 20 nM

STANDARD CURVE RANGE:
2 pM - 200 pM

PRECISION:
Intra-assay 0.8 - 8.5%
Inter-assay 2.9 - 8.3%

SPIKING RECOVERY:
88.2 - 100 %

LINEARITY OF DILUTION:
84 - 120%