Mouse Cytokine TNF-a

This is a one-plex assay kit manufactured by LINCO Research, Inc. to be used for the quantitative determination of the mouse TNF-a. This kit may be used for the analysis of TNF-a in mouse serum or tissue culture samples.

All components are included in each kit (capture antibody immobilized on microspheres, detection antibody, standards, quality controls, streptavidin-PE, wash buffer, assay buffer, 96-well microtiter filter plate, and serum diluent).

ASSAY CONDITIONS:  
2 hour assay for tissue culture, overnight assay for serum/plasma, 25 µl sample volume.

STANDARD CURVE RANGE:  
3.2 - 10,000 pg/ml

SENSITIVITY:  
£ 3 pg/ml 

CROSS REACTIVITY:  
The cytokine antibody pairs in this single plex do not cross react to the other analytes in the multiplexed cytokine panel. 

 

CYTOKINE

Serum
% Recovery

   
TNF-a 96.9
 

PRECISION:  
Intra-assay 14.5%
Inter-assay 8.9%

We will be expanding mouse cytokine panels as we go forward to include additional molecules important to your research needs.

This kit is for research only and not for use as a clinical or diagnostic tool.