Human Intact Proinsulin ELISA Kit
Proinsulin is made by the beta cells of the pancreas. It is later cleaved to form C-peptide and insulin. Proinsulin circulates in small quantities along with insulin and C-peptide due to incomplete modification.
This Intact Proinsulin ELISA kit is used for the non-radioactive quantification of intact human proinsulin in serum and plasma. The kit does not cross-react with human insulin as high as 10 nM. In addition, presence of insulin (up to 208µU/ml) in serum or plasma does not interfere with the assay result. The kit has no cross-reactivity with the major species of proinsulin metabolite, des(31,32) proinsulin, while cross-reacts only weakly with the minor intermediate des(64,65) proinsulin. One kit is sufficient to measure 37 unknown samples in duplicate. This kit is for research purposes only.
ASSAY CONDITIONS:
3 hour assay at room temperature
50 µl sample size.
SENSITIVITY:
The lowest level of Intact proinsulin that can be detected by this assay is 0.1 pM when using a 50 µl sample size.
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STANDARD CURVE RANGE: PRECISION: LINEARITY OF DILUTION:
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