Human Intact Proinsulin ELISA Kit

Proinsulin is made by the beta cells of the pancreas.  It is later cleaved to form C-peptide and insulin.  Proinsulin circulates in small quantities along with insulin and C-peptide due to incomplete modification.

This Intact Proinsulin ELISA kit is used for the non-radioactive quantification of intact human proinsulin in serum and plasma. The kit does not cross-react with human insulin as high as 10 nM. In addition, presence of insulin (up to 208µU/ml) in serum or plasma does not interfere with the assay result. The kit has no cross-reactivity with the major species of proinsulin metabolite, des(31,32) proinsulin, while cross-reacts only weakly with the minor intermediate des(64,65) proinsulin. One kit is sufficient to measure 37 unknown samples in duplicate.  This kit is for research purposes only.

ASSAY CONDITIONS:  
3 hour assay at room temperature
50 µl sample size.

SENSITIVITY: 
The lowest level of Intact proinsulin that can be detected by this assay is 0.1 pM when using a 50 µl sample size.
 

STANDARD CURVE RANGE:
0.5 pM - 100 pM 

PRECISION:
Intra-assay 0.4 - 7.7%
Inter-assay 3.3 - 9.0%

LINEARITY OF DILUTION:
95 - 100%

SPECIFICITY:
Intact Human Proinsulin 100%
Des(31,32) Human Proinsulin 0%
Des(64,65) Human Proinsulin 36%
Bovine Proinsulin 0%
Porcine Proinsulin 0%
Human Insulin 0%
Rat Insulin 0%
Porcine Insulin 0%
Bovine Insulin 0%
Ovine Insulin 0%