Sensitive Rat Insulin RIA Kit
This sensitive rat insulin assay has been developed using the same components as in our regular rat insulin kit (RI-13K) but formated to improve sensitivity by approximately 10-fold. Sample volumes as low as 10 µl can be used for most measurements. The kit includes all components (standards, antibody, tracer, quality controls, precipitating reagents and buffer) necessary to complete a RIA.
ASSAY CONDITIONS: Two-day, disequilibrium incubation at 4°C. Sample volume: £ 100 µl serum, plasma, or tissue culture media.
LABEL: 125 I-Insulin (HPLC purified, 12,500 CPM/tube)
ED80 = 0.07 ± 0.01 ng/ml
ED50 = 0.18 ± 0.01 ng/ml
ED20 = 0.47 ± 0.02 ng/ml