Rat Leptin RIA Kit

This rat leptin assay has been developed to measure rat leptin in plasma or serum. It is a completely homologous assay since the antibody was raised against highly purified rat leptin and both the standard and tracer are prepared with rat leptin. All components are included (standards, antibody, tracer, quality controls, precipitating reagents and buffer) necessary to complete a RIA.

ASSAY CONDITIONS: Two-day, disequilibrium incubation at room temperature. Sample volume: £ 100 µl serum or plasma.

LABEL: 125 I-Rat Leptin (HPLC purified, 18,000 CPM/tube)

 

SPECIFICITY:
Rat Leptin
100%
Mouse Leptin
100%
Human Leptin
<2%
Canine Leptin
0.5%
Insulin
*
Glucagon
*
SRIF
*
 
* Not Detectable

PERFORMANCE:

ED80 = 1.2 ± 0.1 ng/ml
ED50 = 3.6 ± 0.4 ng/ml
ED20 = 11.5 ± 1.3 ng/ml