TGFb1 (Transforming Growth Factor Beta)

Transforming growth factor Beta (TGFβ1) is a multi-functional growth factor regulating many cell functions involved in tissue growth/repair, inflammation, tumor pathogenesis and other normal and disease states. The single-plex TGFβ1 assay kit can be used to quantify human, mouse, rat, and porcine TGFβ1 in serum and cell/tissue culture samples. Since this kit requires sample pretreatment,TGFβ1 cannot be multiplexed with other analytes. This LINCOplex® kit is to be used with Luminex100® systems, powered by xMAP™ Technology or related platforms.

The antibody pair used recognizes human, mouse, rat, and porcine TGFβ1. The kit has been validated for human serum and tissue culture. However, since TGFβ1 is conserved across various species (bovine, swine, canine, rat, mouse, hamster), this kit may be applicable to measure TGFβ1 in the above species.

 

ASSAY CONDITIONS:  
  25 µL (sample pretreatment required)
  3.5 hour or overnight assay

STANDARD CURVE RANGE:  
   .064 - 200 ng/mL

SENSITIVITY :
20 pg/mL (serum-free)
60 pg/mL (serum)

CROSS REACTIVITY:  
There is no crossreactivity with other known cytokines

PRECISION:  
Intra-assay 6.1%
Inter-assay 9.2%

ACCURACY:  
97%