Human Cytokine/Chemokine LINCOplex Kit

Cytokine is a generic name used for a diverse group of soluble proteins and peptides which act as humoral regulators either under normal or pathological conditions to modulate the functional activities of individual cells and tissues. These proteins also mediate interactions between cells directly and regulate processes taking place in the extracellular environment. Cytokines differ from
hormones in that they act on a wider spectrum of target cells and also, unlike hormones, they are not produced by specialized cells which are organized in specialized glands. This group of proteins include lymphokines, interferons, colony stimulating factors and chemokines.

The LINCOplex® line of kits enables simultaneous multi-analyte detection and measurement of Human Cytokines and Chemokines.You may customize your assay kit by selecting any combination of the cytokines/chemokines or the entire panel. LINCOplex® is especially useful for conducting multiplex assays on samples where limited volumes are available.

All components are included in each kit (capture antibody immobilized on microspheres, bead diluent, detection antibody, standards, quality controls, streptavidin-PE, wash buffer, assay buffer, 96-well microtiter filter plate, serum matrix) and are sufficient to run 39 samples (serum, plasma or tissue culture medium) in duplicate in a 96-well microtiter plate.

ASSAY CONDITIONS: 25 μL sample volume
                                         2 hour or overnight assay

STANDARD CURVE RANGE: 3.2 - 10,000 pg/mL

SENSITIVITY IN TISSUE CULTURE/SERUM/PLASMA: 0.5 - 16 pg/mL; Refer to kit protocol for sensitivities for individual cytokines/chemokines.

CROSS REACTIVITY: The cytokine antibody pairs in this multiplex assay do not cross react with other analytes in the panel.

ACCURACY:  100 ± 20% for all analytes

PRECISIONIntra-assay 4.8 - 9.0%
                        Inter-assay 3.1 - 18.4%
 



 

ANALYTE
AVAILABILITY:
You may customize
your panel. Order any
combination of:
 

PREMIXED PANELS



 

13- PLEX

21/22-PLEX

29/30-PLEX

IL-1α
IL-1β
IL-1ra
IL-2
IL-4
IL-5
IL-6
IL-7
IL-8
IL-10
IL-12(p40)
IL-12(p70)
IL-13
IL-15
IL-17
EGF
Eotaxin
Fractalkine
G-CSF
GM-CSF
IFN-γ
IP-10
MCP-1
MIP-1α
MIP-1β
RANTES†
sCD40L
TGFα
TNFα
VEGF

IL-1β
IL-2
IL-4
IL-5
IL-6
IL-7
IL-8
IL-10
IL-12(p70)
IL-13
GM-CSF
IFN-γ
TNF-α
IL-1α
IL-1β
IL-2
IL-4
IL-5
IL-6
IL-7
IL-8
IL-10
IL-12(p70)
IL-13
IL-15
IL-17
Eotaxin
G-CSF
GM-CSF
IFN-γ
IP-10
MCP-1
MIP-1α
TNF-α

RANTES
IL-1α
IL-1β
IL-1ra
IL-2
IL-4
IL-5
IL-6
IL-7
IL-8
IL-10
IL-12(p40)
IL-12(p70)
IL-13
IL-15
IL-17
EGF
Eotaxin
Fractalkine
G-CSF
GM-CSF
IFN-γ
IP-10
MCP-1
MIP-1α
MIP-1β
sCD40L
TGFα
TNFα
VEGF

RANTES


†Serum/plasma samples typically contain high levels of RANTES, and must be diluted for accurate measurement. For single plex RANTES measurements, use kit #HCYTO-60K-1RNTS. RANTES available for addition to 21-PLEX and/or 29-PLEX premix panel (for tissue culture supernatants) upon request.

View Linco Protocol

Related Kits:

HCYTO-60K-1TNFA