Elisa test kits
Indirect competitive 96 well ELISA Assays are the most used for the quantification of very small substances, such as hormones (Jordan, 2005).
The basis of the technique lies in the competition between the conjugated form of the antigen with the samples for the binding sites to the antibody, the binding of these to a second enzymatic antibody can be detected using a suitable substrate that produces color.
Competition between the conjugated antigen and the sample interferes with the ability of the enzyme antibody to bind to the captured monoclonal antibody, so the reading on a spectrophotometer is inversely proportional to the amount of antigen in the sample (Asch, 2000).
The first applications of this technique for the quantification of ABA were made by Quarrie et al . (1988), who determined that the monoclonal antibody MAC 62 (MAC 252) turns out to be the most specific.
Cahill & Ward (1989) rated this method as specific and reliable for the detection of ABA in raw soybean tissue extracts.
It has been established that the most widely used carrier protein that provides satisfactory results is Bovine Serum Albumin-BSA (Jinqing et al ., 2011).
Lastly, kits have been implemented for the analysis of this phytohormone; however, most of these kits have two disadvantages: the poor stability of the results obtained and the high cost.
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