Mouse Endocrine Immunoassay Panel

This multiplex assay kit manufactured by LINCO Research, Inc. can be used for the simultaneous quantitative determination of the following five rat and mouse endocrine hormones in any combination:  Insulin, Amylin, Leptin, Glucagon, and GLP-1.  LINCOplexTM is especially useful for conducting multiplex assays on samples where limited volumes are available.

All components are included in each kit (capture antibody immobilized on microspheres, bead diluent, detection antibody cocktail, standards, cocktail quality controls, streptavidin-PE, wash buffer, assay buffer, 96-well microtiter filter plate, serum matrix) and are sufficient to run 38 samples (serum, plasma or tissue culture medium) in duplicate in a 96-well microtiter plate..

 

ASSAY CONDITIONS:  
10 µL sample volume, Overnight assay. 

STANDARD CURVE RANGE:  
6.2 - 4,500 pM

SENSITIVITY IN TISSUE CULTURE:
£ 6.2 pM

CROSS REACTIVITY:  
Each antibody pair used in this panel is highly specific and does not show any significant cross-reactivity to other analytes within this panel.

PRECISION:  
Intra-assay 3.8 - 10.6%
Inter-assay 4.8 - 20.7%

LINEARITY OF DILUTION:  
79 - 100%
ENDOCRINE HORMONES

Recovery in Serum Matrix

Recovery in Serum Samples
Insulin 100.3% 105.8%
Amylin 101.0% 80.2%
Leptin 100.5% 96.5%
Glucagon 99.7% 105.9%
GLP-1 101.1% 115.2%


We will be expanding these endocrine panels as we go forward to include additional molecules important to your research needs.

 

This kit is for research only and not for use as a clinical or diagnostic tool.