Human CVD Biomarker Panel 1

This multiplex assay kit manufactured by LINCO Research can be used for the simultaneous quantification of the following analytes in any combination: Adiponectin (Acrp30), soluble E-Selectin (sE-Selectin), soluble VCAM-1 (sVCAM-1), soluble ICAM-1 (sICAM-1), Matrix Metalloproteinase-9 (MMP-9), Myeloperoxidase (MPO), and Total Plasminogen Activator Inhibitor-1 (tPAI-1).This kit can be used for the analysis of the above analytes in tissue extract, cell/tissue culture samples, and diluted
human serum or plasma samples. Adiponectin, sVCAM-1, MMP-9, MPO, and tPAI-1 have detectable cross reacting signals in non-human primate serum or plasma samples. Call for additional information.

ASSAY CONDITIONS:
25μL sample volume
Overnight assay
1:100 dilution (1:50 if adiponectin is not measured) for both serum and plasma samples

STANDARD CURVE RANGE:
16 to 50,000 pg/ml for MMP-9, MPO and total PAI-1
80 to 250,000 pg/ml for sE-Selectin, sICAM-1, sVCAM-1, and Adiponectin

SENSITIVITY IN
TISSUE CULTURE/SERUM/PLASMA (MinDC*):

sE-Selectin                 79 pg/ml                                 
sVCAM-1                 16 pg/ml
sICAM-1                    9 pg/ml
MMP-9                       1 pg/ml
MPO                           7 pg/ml
Adiponectin                56 pg/ml
tPAI-1                          1 pg/ml

* Minimum Detectable Concentration

CROSS REACTIVITY:
No detectable cross reactivity within the panel    

ACCURACY:
sE-Selectin                104.1%
sVCAM-1                103.9%
sICAM-1                   83.0%
MMP-9                     91.1%
MPO                         91.7%
Adiponectin               96.4%
tPAI-1                       84.6%

PRECISION:
Intra-assay           4.5 – 12.3%
Inter-assay           8.5 – 16.3%

LINEARITY OF DILUTION:
89.4 - 118.4%