Mouse Leptin RIA Kit

This mouse leptin assay has been developed to measure mouse leptin in plasma or serum. It is a completely homologous assay since the antibody was raised against highly purified mouse leptin and both the standard and tracer are prepared with mouse leptin. All components are included (standards, antibody, tracer, quality controls, precipitating reagents and buffer) necessary to complete a RIA.

ASSAY CONDITIONS: Two-day, disequilibrium incubation at 4°C. Sample volume: £ 100 µl serum or plasma.

LABEL: 125I-Mouse Leptin (HPLC purified, 18,000 CPM/tube).

 

SPECIFICITY:
Mouse Leptin
100%
Rat Leptin 50%
Human Leptin
<0.2%
Canine Leptin
*
Human Insulin
*
Human C-Peptide
*
IGF-I
*
SRIF
*
Glucagon
*
Somatostatin
*
Pancreatic Polypeptide
*
* Not Detectable

PERFORMANCE:

ED80 = 0.7 ± 0.1 ng/ml
ED50 = 2.4 ± 0.2 ng/ml
ED20 = 8.7 ± 0.9 ng/ml