Glucagon Like Peptide-1 (Total) RIA Kit

This assay specifically measures biologically active glucagon-like peptide-1 (GLP-1) (7-36) in alcohol extracted plasma from many species. The kit includes all components (standards, matrix, antibody, tracer, quality controls, precipitating reagent and buffers) necessary to complete a RIA.

ASSAY CONDITIONS: Two-day, disequilibrium assay at 4°C. Sample volume = 300 µl plasma. (Extraction required)

LABEL: 125 I-GLP-1 (monoiodinated, HPLC purified, 20,000 CPM/tube).

SPECIFICITY:
GLP-1 (7-36) (Human) 100%
GLP-1 (7-37) (Human) 100%
GLP-1 (9-36) (Human) <0.1%
GLP-2 (Human) *
Glucagon (Human) 16%
Insulin (Human) *
GIP (Human)  *
Secretin (Human) *
VIP (Human, Porcine, Rat) *
* Not Detectable

PERFORMANCE:

ED80 = 28 ± 7 pM
ED50 = 82 ± 10 pM
ED20 = 241 ± 24 pM