Human Sepsis/Apoptosis LINCOplex Kit

This multiplex assay kit manufactured by LINCO Research is to be used for the simultaneous quantification of the following analytes in any combination: soluble VCAM-1 (sVCAM-1), soluble ICAM-1 (sICAM-1), soluble Fas Ligand (sFasL), soluble Fas (sFas), Macrophage Migration Inhibitory Factor (MIF) and Total Plasminogen Activator Inhibitor-1 (PAI-1). This kit is fully validated for the analysis of the above analytes in tissue extract, cell/tissue culture samples, and diluted human serum or plasma samples. The analytes sVCAM-1, MIF and tPAI-1 show cross-reactivity with monkey serum samples.  This kit is for research purposes only.

All components are included in each kit (capture antibody immobilized on microspheres, detection antibody cocktail, standards cocktail, quality controls, streptavidin-PE, wash buffer, assay buffer, 96-well microtiter filter plate) in sufficient quantity to run 39 samples in a 96-well microtiter plate. 

ASSAY CONDITIONS:  
Sample size: 25 µL

CROSS REACTIVITY:  
There is no detectable crossreactivity within the panel.

STANDARD CURVE RANGE:  
61 - 250,000 pg/mL for sVCAM-1 and sICAM-1
12 - 50,000 pg/mL for sFas, sFasL, MIF and PAI-1
 
ACCURACY:
Analyte Serum
sVCAM-1 98
sICAM-1 87
sFas 71
sFasL 89
MIF 79
PAI-1 89

SENSITIVITY:
(Minimum Detectable Concentrations,
MinDC):
Analyte

MinDC
(pg/mL)

 
sVCAM-1 33.0  
sICAM-1 30.0  
sFas 7.0  
sFasL 6.0  
MIF 10.0  
PAI-1 3.1

 

PRECISION:  
Intra-assay variation: 3.5 - 9.5%
Inter-assay variation: 6.4 - 14.2%

LINEARITY OF DILUTION:
Analyte Dilution 1:8 Dilution 1:16

Dilution 1:32
sVCAM-1 95.6 94.9 88.2
sICAM-1 100.9 109.6 109.2
sFas 100.7 106.1 112.5
sFasL 103.4 105.6 114.5
MIF 110.6 116.7 120.0
PAI-1 - 117.8 134.3