Glucagon RIA Kit
A sensitive and reproducible assay for measurement of pancreatic glucagon (in serum or plasma) in most mammals. Crossreactivity to enteric glucagon is less than 0.1%. All components are included (standards, antibody, tracer, quality controls, precipitating reagents and buffer) necessary to complete a RIA.
ASSAY CONDITIONS: Two-day, disequilibrium incubation at 4°C. Sample volume: £ 100 µl serum, plasma, or tissue culture medium.
LABEL: 125 I-Glucagon (HPLC purified, 19,000 CPM/tube)
ED80 = 39 ± 4 pg/ml
ED50 = 104 ± 8 pg/ml
ED20 = 271 ± 21 pg/ml