Glucagon Like Peptide-1 (Active) ELISA Kit

This assay has been developed to measure biologically active glucagon-like peptide-1 (7-36, 7-37) in plasma, serum or tissue culture medium. It is a two-site non-competitive immunoassay based on enzyme labeled quantification of GLP-1 detected by a fluorogenic substrate. All components are included (standards, enzyme labeled antibody, quality controls, wash buffer, assay buffer, substrate and stop solutions) necessary to complete a 96-well plate ELISA.

NON-EXTRACTION ASSAY

ASSAY CONDITIONS:Overnight incubation at 4°C, followed by 2 hours 30 minutes at room temperature. Sample volume must be less than or equal to 100 µl serum, plasma or tissue culture medium.

 

SPECIFICITY:
GLP-1 (7-36 Amide)
100%
GLP-1 (7-37)
100%
GLP-1 (9-36 Amide)
*
GLP-2
*
Glucagon
*
* Not Detectable


PERFORMANCE:

ED80 = 81 ± 4 pM
ED50 = 55 ± 7 pM
ED20 = 28 ± 1 pM