Human Gastric Inhibitory Peptide ELISA
* All components are included (standards, quality controls, serum matrix, wash buffer, assay buffer, substrate and stop solutions, capture antibody immobilizedplate, enzyme linked streptavidin, and detection antibody) necessary to complete a 96-well plate ELISA.
GIP, also known as gastric inhibitory polypeptide, or glucose-dependent insulinotropic polypeptide, is a 42 amino acid peptide hormone synthesized in and secreted from K cells in the intestinal epithelium. There are two major GIP molecular forms in circulation, GIP(1-42) and GIP(3-42). Previous studies have demonstrated that GIP(3-42) is a degraded form of GIP(1-42) by the enzyme DPPIV. GIP secretion is primarily regulated by nutrients, especially fat. GIP exhibits potent incretin activity in rodents and human subjects. The primary action of GIP is the stimulation of glucose-dependent insulin secretion. GIP may also play a role in adipocyte biology. Recent studies found that the inhibition of GIP signaling prevents the onset of obesity and consequent insulin resistance induced by a high fat diet.
This ELISA measures total GIP, i.e. GIP(1-42) and GIP(3-42).
ASSAY CONDITIONS: Room temperature, 3.5 hour assay, 20 μl sample volume, serum, plasma, or tissue culture medium
STANDARD CURVE RANGE: 8.2-2000 pg/ml
SENSITIVITY IN TISSUE CULTURE/SERUM/PLASMA: 8.2 pg/ml
* Not detectable ACCURACY: 86.7 ± 3.0% PRECISION: Intra-assay 3.0 – 8.8 % LINEARITY OF DILUTION: 99.9 ± 2.7 %
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