Human Insulin ELISA Kit

This Human Insulin ELISA has been developed to measure human insulin in serum, plasma or tissue culture media.  It is a two-site immunoassay using two monoclonal antibodies.  Signal is derived by monitoring (450 nm) horseradish peroxidase activity in the presence of the substrate 3,3',5,5'-tetramethylbenzidine.  This method offers an alternative non radioactive immunoassay for laboratories interested in reducing use of radioisotopes.

All components are included (standards, antibody, enzyme conjugate, quality controls, wash buffer, assay buffer, substrate and stop solutions) necessary to measure 38 unknown samples in duplicate on a 96-well ELISA plate.

ASSAY CONDITIONS: Less than 2 hour, room temperature incubation. Sample volume: £ 20 µl serum, plasma, or tissue culture media.

SPECIFICITY:
Human Insulin 100%
Porcine Insulin 154%
Bovine Insulin 56%
Ovine Insulin 39%
Rat Insulin *
Human Proinsulin **
Des(64,65) Human Proinsulin 117%
Des(31,32) Human Proinsulin 0.3%
Porcine Proinsulin <0.1%
Bovine Proinsulin < 0.1%
Human IGF-I *
Human IGF-II *
Glucagon *
Glucagon-like Peptide 1 **
Human C-Peptide *
Rat C-Peptide *
Human Leptin *
Rat Leptin *
Mouse Leptin *
 


* Not detectable at concentrations up to 120 nM
** Not detectable at concentrations up to 100 nM

PERFORMANCE:  

ED50 = 0.68 M