Type IIA Collagen N-Propeptide (PIIANP) ELISA KIT
All components are included (standards, quality controls,wash buffer, assay buffer, substrate, stop solution, anchor antibody immobilized plate, antibody PIIANP, biotinylated PIIANP, and enzyme linked streptavidin) necessary to complete a 96-well plate ELISA.
Type II collagen is the major collagen found in cartilage and is expressed in two forms: IIA and IIB. Type IIA procollagen contains an N-terminal 69 amino acid, cysteine-rich globular domain that is encoded by exon 2. Type IIB procollagen is synthesized by mature chondrocytes in cartilage while Type IIA procollagen is synthesized by chondroprogenitor cells. The Type IIA N-propeptide (PIIANP) has been postulated to play a role in chondrogensis. Type IIA procollagen has been found to be synthesized by osteoarthritic chondrocytes in diseased cartilage and may serve as a specific arthritis biomarker that reflects an attempt by the chondrocytes to repair the diseased cartilage.
|ASSAY CONDITIONS: Room temperature, 3 hour assay 50 µL sample size (samples diluted 1:5 for assay)
DYNAMIC RANGE: 4.7 – 300 ng/mL
SENSITIVITY IN SERUM: 1.1 ng/mL
SPECIFICITY: Human PIIANP 100%
PRECISION: Intra-assay 3.4 – 6.6%
LINEARITY OF DILUTION: 91-109%