Porcine/Canine Growth Hormone RIA Kit

Growth Hormone is secreted from the anterior pituitary and its measurement in circulation reflects pituitary function particularly of somatotrophs.  Growth hormone influences both whole body metabolism and growth.  This Porcine / Canine Growth Hormone radioimmunoassay has been developed to quantitate Growth Hormone in plasma, serum, and tissue culture media. It is a completely homologous assay since the antibody was raised against recombinant Porcine Growth Hormone and both the standard and tracer are prepared with recombinant Porcine Growth Hormone. Since the amino acid sequences of Porcine Growth Hormone and Canine Growth Hormone are identical, this assay developed for Porcine Growth Hormone measures Canine Growth Hormone levels with equal efficiency. All components are included (standards, antibody, tracer, quality controls, precipitating reagents and buffer) necessary to complete a RIA.

ASSAY CONDITIONS: Overnight, equilibrium incubation at room temperature. Sample volume: £ 100 µl serum, plasma, or tissue culture media.

LABEL: 125I-Porcine/Canine Growth Hormone (18,000 CPM/tube).



ED80 = 2.3 ± 0.2 ng/ml
ED50 = 9.8 ± 0.5 ng/ml
ED20 = 41.8 ± 1.4 ng/ml

Porcine Growth Hormone 100%
Canine Growth Hormone 100%
Porcine Prolactin <0.1%
Human Growth Hormone <0.5%