Human Endocrine Immunoassay Panel
Adipose tissue is not only a site of energy storage, but also an active endocrine organ that secretes a number of horomones and cytokines including leptin, resistin, adiponectin, IL-1b, IL-6, TNFa, MCP-1, PAI-1 and others. These adipocyte-derived proteins (adipocytokines or adipokines) play critical roles in body energy homeostasis and metabolism. LINCO has developed the following multiplex immunoassays for simultaneous quantitative determination of several adipokines in rat serum/plasma or adipocyte samples, using Luminex xMAP technology.
All components are included in each kit (capture antibody immobilized on microspheres, detection antibody cocktail, standard cocktail, quality controls, streptavidin-PE, wash buffer, assay buffer, 96-well microtiter filter plate, etc.) in sufficient quantity to run 39 samples in a 96-well microtiter plate.
1) Rat Adipocyte Panel (Catalog No. RADPCYT-82K): This kit is used to measure Leptin, IL-1b, IL-6, TNFa, MCP-1, and PAI-1 (active or total) in adipocyte extracts or culture medium samples.
2) Rat Adipokine Panel (Catalog No. RADPK-81K): This kit is used to measure Leptin, IL-1b, IL-6, TNFa, MCP-1, PAI-1 (active or total) and Insulin in rat serum or plasma samples.
ASSAY CONDITIONS:
25μL sample volume
Overnight assay
STANDARD CURVE RANGE:
4.9-20,000 pg/mL IL-1b, TNFa, MCP-1
12.2-50,000 pg/mL Leptin, IL-6, PAI-1 (active or total), and Insulin
CROSS REACTIVITY:
Each antibody pair in a panel is highly specific and displays insignificant cross-reactivity to other analytes in the panel.
SENSITIVITY (Minimum Detectable Concentrations, MinDC, pg/mL):
|
Adipocyte Panel |
Serum Adipokine Panel |
|
| Leptin | 4.6 | 9.7 |
| IL-1b | 1.4 | 1.2 |
| IL-6 | 3.0 | 8.8 |
| TNFa | 0.8 | 3.2 |
| MCP-1 | 23.0 | 25.8 |
| PAI-1 (active) | 31.3 | 31.6 |
| PAI-1 (total) | 29.2 | 30.7 |
| Insulin | - | 52.5 |
ACCURACY/RECOVERY (%):
|
Adipocyte Panel |
Serum Adipokine Panel |
||
| Leptin | 91 | 103 | |
| IL-1b | 95 | 103 | |
| IL-6 | 84 | 106 | |
| TNFa | 100 | 116 | |
| MCP-1 | 91 | 106 | |
| PAI-1 (active) | 88 | 110 | |
| PAI-1 (total) | 93 | 98 | |
| Insulin | - | 107 |
PRECISION:
|
Adipocyte Panel |
Serum Adipokine Panel |
|
| Intra-assay | <6 | <4 |
| Inter-assay | <23 | <14 |